O-234 Constant antioxidant supplementation increases blastocyst formation from oocyte donors

Abstract Study question Does constant supplementation of antioxidants to the culture media increase blastocyst formation from oocyte donors? Summary answer The increases donors What is known already use a combination various in embryo has recently been investigated explore potential benefit for development and clinical outcomes. However, concentration added only determined by its effect. In this study, we performed repeated resemble physiological oxidation reduction environment. Therefore, effect every 12 hours on expansion donors. design, size, duration This prospective study was conducted at CITMER, Mexico April 2020 November 2022.We included total 258 recipients undergoing IVF/ICSI. Participants/materials, setting, methods A 2403 zygotes were divided into 4 groups cultured following conditions until stage: Group 1A: 563 20% O2 with hours, 1B: 1109 beginning, 2A: 339 5% 2B: 392 beginning. Embryo assessed. Odds ratio Fisher test performed. p < 0.05=significant Main results role chance For both oxygen tensions, found significant rate (day 5+day 6) when repeatedly (1A: 54.7% vs 45.9%, = 0.0007*; 58.7% 46.9%, 0.001*). addition, days 5 + 6 also significantly higher than where beginning 35.9% 29.9%, 0.01*; 41.3% 31.6%, 0.006*). Limitations, reasons caution Given sibling patients are their own controls. Wider implications findings Constant as well rate, which may improve Trial registration number 06162020-aox04